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血根碱对LPS诱导后黄鳝免疫应激及肠道炎症相关基因表达的影响
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作者:石勇1  胡毅1 2  刘艳莉1  车常宝1  钟蕾1 2 
单位:1. 湖南农业大学湖南省特色水产资源利用工程技术研究中心, 湖南 长沙 410128;
2. 湖南农业大学中兽药湖南省重点实验室, 湖南 长沙 410128
关键词:黄鳝 血根碱 脂多糖 免疫 基因表达 
分类号:S941
出版年·卷·期(页码):2020·27·1(125-136)
摘要:
为探讨血根碱是否具有缓解黄鳝(Monopterus albus)由脂多糖(LPS)诱导后引起的免疫应激及炎症反应作用,本实验设置对照组(基础饲料)和实验组(基础饲料+750 μg/kg血根碱),每组3个重复,养殖实验持续8周,结束后每个重复随机取30尾规格一致的黄鳝经腹腔注射大肠杆菌脂多糖(LPS),在注射前0 h和注射后3 h、6 h、12 h、24 h和48 h进行采样。结果显示,在0 h时,实验组肝脏中超氧化物歧化酶(SOD)活力显著高于对照组(P<0.05),其余指标均无显著性差异(P>0.05);血清中葡萄糖(GLU)、皮质醇(COR)、IgM、C4、碱性磷酸酶(AKP)、谷草转氨酶(GOT)、谷丙转氨酶(GPT)和肝脏中SOD在腹腔注射LPS后均呈先上升后下降的趋势,并在3 h或6 h达到峰值,肝脏中丙二醛(MDA)的变化趋势与其相反;与对照组相比,实验组血清中AKP、IgM、C4的含量和肝脏中SOD活力在注射LPS后上升幅度更大,GLU和COR的含量上升幅度慢,并且GLU、COR、AKP、IgM、C4、GOT和GPT恢复至正常水平速度更快。肠道中炎症相关基因IL-1β、IL-10、IL-12β、IL-15、TGF-β1TGF-β2表达量在腹腔注射LPS后呈先上升后下降的趋势,并在6 h或12 h达到峰值;与对照组相比,实验组下调了IL-1βIL-12β基因表达量,上调了IL-10、IL-15、TGF-β1、TGF-β2TGF-β3基因表达量。综上所述,在本研究条件下,腹腔注射LPS诱导黄鳝产生了免疫应激及炎症反应,在3~6 h时出现高峰,而饲料中添加750 μg/kg血根碱能够有效改善黄鳝由LPS诱导产生的免疫应激及炎症反应。
The aim of this study was to investigate the effects of sanguinarine on alleviating the immune stress and inflammation induced by lipopolysaccharide (LPS) in the rice field eel (Monopterus albus). The control (basal diet) and experimental groups (basal diet+750 μg/kg sanguinarine) were set, with 3 replicates in each group. After 8 weeks, 30 eels with consistent specifications were randomly selected from each repeat for the intraperitoneal injection of LPS. Samples were taken 0 h before injection and 3 h, 6 h, 12 h, 24 h, and 48 h after injection. The results showed that at 0 h, superoxide dismutase (SOD) activity in the liver of the experimental group was significantly higher than that of the control group, and there were no significant differences in the other indices. In the serum, glucose (GLU), cortisol (COR), immunoglobulin M (IgM), complement 4 (C4), alkaline phosphatase (AKP), glutamic-oxaloacetic transaminase (GOT), glutamic-pyruvic transaminase (GPT), and SOD in the liver all showed a trend of first increasing and then decreasing after the injection of LPS, and reached a peak at 3 h or 6 h, while the malondialdehyde (MDA) in the liver showed the opposite trend. Compared with the control group, the contents of AKP, IgM, C4 in the serum, and SOD activity in the liver of the experimental group increased more after the LPS injection, while the contents of GLU and COR increased more slowly, and the GLU, COR, AKP, IgM, C4, GOT, and GPT recovered to normal levels more quickly. The expression levels of the IL-1β, IL-10, IL-12β, IL-15, TGF-β1, and TGF-β2 genes in the intestinal tract first increased and then decreased after the injection of LPS, and reached a peak at 6 h or 12 h. Compared with the control group, the expression levels of IL-1β and IL-12β were down-regulated in the experimental group, while the expression levels of IL-10, IL-15, TGF-β1, TGF-β2, and TGF-β3 were up-regulated. Taken together, the findings of the study indicate that under the conditions of this experiment, the injection of LPS induced immune stress and the inflammatory response in the rice field eel, which peaked at 3-6 hours. Furthermore, the addition of 750 μg/kg of sanguinarine into the feed could effectively relieve the immune stress and inflammatory response induced by LPS.
该文献标准引用格式:
SHI Yong, HU Yi, LIU Yanli, CHE Changbao, ZHONG Lei,.Effects of sanguinarine on immune and intestinal inflammation related to gene expression in rice field eels (Monopterus albus) induced by LPS[J].Journal of Fishery Sciences of China,2020,27(1):125-136.[石勇, 胡毅, 刘艳莉, 车常宝, 钟蕾,.血根碱对LPS诱导后黄鳝免疫应激及肠道炎症相关基因表达的影响[J].中国水产科学,2020,27(1):125-136.]
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