首页期刊介绍征稿简则下载专区期刊征订电子期刊联系我们帮助English
 
河南省小黄黝鱼群体遗传多样性
Html全文阅读】 【文章下载
下载次数:192次
作者:汪曦  曾会玲  孟晓林  王先锋  宋东蓥  张建新  顾钱洪  汤永涛  周传江  聂国兴 
单位:河南省水产动物养殖工程技术研究中心, 水产动物疾病控制河南省工程实验室, 河南师范大学水产学院, 河南 新乡, 453007
关键词:河南省 小黄黝鱼 COI基因 遗传多样性 群体遗传结构 
分类号:S931
出版年·卷·期(页码):2020·27·1(96-105)
摘要:
小黄黝鱼[Micropercops swinhonis(Günther,1873)]是中国特有的一种小型淡水虾虎鱼类,广泛分布于长江及以北各水系,其中在河南省各水系均有分布。为评估河南省小黄黝鱼种质资源现状及其遗传多样性,本研究对河南省境内黄河、长江、淮河和海河4个水系77尾小黄黝鱼样品进行线粒体COI基因的扩增。结果显示,河南省小黄黝鱼的平均单倍型多样性(Hd)为0.89088,平均核苷酸多样性(π)为0.00361,其中淮河水系华行村种群(HXC)核苷酸多样性最高(π=0.00433),黄河水系马颊河种群(MJH)核苷酸多样性最低(π=0.00078);系统发育树和单倍型网络图结果均显示小黄黝鱼并未按照水系形成明显的地理遗传支系;根据4个水系进行分组,AMOVA分析显示仅16.00%的变异来自于水系间,而78.43%遗传变异来自各群体内部;同样地,未预先设定分组情况的SAMOVA分析也得到了相似的结果,不支持小黄黝鱼具有明显的地理遗传结构的假设;种群历史动态分析显示,河南省小黄黝鱼种群呈逐步增长趋势;Fu's FS中性检验与错配分析表明,河南小黄黝鱼种群可能经历过近期群体扩张事件,且该过程可能与气候变为暖湿型有关。
Micropercops swinhonis is a specific small freshwater demersal fish, which is widely distributed in the water systems of the Yangtze River and its northern rivers, as well as the rivers of Henan Province. To assess the current situation of germplasm resources and genetic diversity, 77 M. swinhonis samples were collected from the basins of the Yellow River, Yangtze River, Huaihe River, and Haihe River in Henan Province. We analyzed the population genetic diversity and differentiation of the species using the mitochondrial COI gene. In this study, we amplified the partial mitochondrial COI gene sequences of 77 individuals from 10 populations (PH, BSH, HZH, TRQ, MJH, XLSSK, HXC, YH, QH, and WH). The results showed an average haplotype diversity of 0.89088 and average nucleotide diversity of 0.00361 in all populations, with a maximum and minimum in the HXC and NJH populations, respectively. AMOVA analysis showed that the genetic differentiation of M. swinhonis mainly occurred within the population variation, with 78.43% genetic variation within M. swinhonis populations. The results of phylogenetic tree and network of haplotypes suggested that M. swinhonis did not form from a significant geographic cluster. Similarly, the results of SAMOVA support the phylogenetic tree and network of haplotypes when not using grouping in advance. Demographic history analysis showed that the 10 populations experienced rapid population growth and have maintained an upward trend. Fu's FS value and mismatch nucleotide analysis revealed that a recent population expansion had occurred in M. swinhonis populations, the process for which may be related to the climate changing to warm and humid.
该文献标准引用格式:
WANG Xi, ZENG Huiling, MENG Xiaolin, WANG Xianfeng, SONG Dongying, ZHANG Jianxin, GU Qianhong, TANG Yongtao, ZHOU Chuanjiang, NIE Guoxing.Genetic diversity of wild Micropercops swinhonis populations in Henan Province[J].Journal of Fishery Sciences of China,2020,27(1):96-105.[汪曦, 曾会玲, 孟晓林, 王先锋, 宋东蓥, 张建新, 顾钱洪, 汤永涛, 周传江, 聂国兴.河南省小黄黝鱼群体遗传多样性[J].中国水产科学,2020,27(1):96-105.]
参考文献:
[1] Wu H L, Zhong H S. Faunica Sinica, Osteichthyes, Perciformes (V) Gobioidei[M]. Beijing:Science Press, 2008:141-145.[伍汉霖, 钟汉生. 中国动物志硬骨鱼纲鲈形目(五)虾虎鱼亚目[M]. 北京:科学出版社, 2008:141-145.]
[2] Kim I S, Kim B J. Population ecology of the goby, Micropercops swinhonis in the Puan-gun, Ch'ollabuk-do, Korea[J]. Korean Journal of Limnology, 1997, 30:47-54.
[3] Kim I S. Breeding habits and egg development of the goby, Micropercops swinhonis[J]. The Korean Journal of Ecology, 1996, 19(5):477-486.
[4] Iwata A, Sakai H, Shibukawa K, et al. Developmental characteristics of a freshwater goby, Micropercops swinhonis, from Korea[J]. Zoological Science, 2001, 18(1):91-97.
[5] Nichol J T. The freshwater fishes of China[J]. Nature History of Central Asia, 1943, 9:258-259.
[6] Yi B L. Notes on species of genus Hypseleotris (Eleotridae) with respect to its sex dimorphism[J]. Journal of Huazhong Agricultural College, 1982, 1(3):72-77.[易伯鲁. 黄黝鱼属的种类及其两性异形[J]. 华中农学院学报, 1982, 1(3):72-77.]
[7] Zheng X, Li C H. Population structure and genetic divergence of Micropercops swinhonis[J]. Journal of Shanghai Ocean University, 2017, 26(3):358-365.[郑新, 李晨虹. 中国小黄黝鱼种群遗传结构和分化研究[J]. 上海海洋大学学报, 2017, 26(3):358-365.]
[8] Amor M D, Norman M D, Cameron H E, et al. Allopatric speciation within a cryptic species complex of Australasian octopuses[J]. PLoS ONE, 2014, 9(6):e98982.
[9] Hu J, Hou X Y, Yin S W, et al. Genetic diversity and divergence of cheilinus undulatus of different geographic populations of the South China Sea revealed by COI and Cyt b gene analyses[J]. Acta Hydrobiologica Sinica, 2014, 38(6):1008-1016.[胡静, 侯新远, 尹绍武, 等. 基于mtDNA COI和Cyt b基因序列对南中国海不同海域波纹唇鱼群体遗传多样性的研究[J]. 水生生物学报, 2014, 38(6):1008-1016.]
[10] Shan Y J, Lu C Y, Li C, et al. Study of DNA barcoding and genetic variation based on the mitochondrial COI gene sequences in five carp varieties[J]. Journal of Fishery Sciences of China, 2013, 30(5):931-938.[单云晶, 鲁翠云, 李超, 等. 基于线粒体COI基因序列的5种鲤养殖品种遗传多样性研究[J]. 中国水产科学, 2013, 30(5):931-938.]
[11] Zhang Y, Zhou C J, Gu Q H, et al. Molecular phylogenetic analysis of gobies in the north of Henan based on COI and D-Loop sequences[J]. Journal of Henan Normal University (Natural Science Edition), 2016, 44(5):94-100.[张毅, 周传江, 顾钱洪, 等. 基于COI和D-Loop序列豫北虾虎鱼分子系统分析[J]. 河南师范大学学报(自然科学版), 2016, 44(5):94-100.]
[12] Liang H W, Meng Y, Luo X Z, et al. Genetic diversity of six Monopterus albus populations based on COI gene sequences[J]. Journal of Fishery Sciences of China, 2018, 25(4):837-846.[梁宏伟, 孟彦, 罗相忠, 等. 基于线粒体COI基因的6个黄鳝群体遗传多样性[J]. 中国水产科学, 2018, 25(4):837-846.]
[13] Sambrook J, Fritsch E F, Maniatis T. Molecular Cloning:A Laboratory Manual[M]. New York:Cold Spring Harbor Laboratory Press, 1989:1626.
[14] Hubert N, Hanner R, Holm E, et al. Identifying Canadian freshwater fishes through DNA barcodes[J]. PLoS ONE, 2008, 3(6):e2490.
[15] Swindell S R, T N Plasterer. Seqman. Contig assembly[J]. Methods in Molecular Biology, 1997, 70(1):75-89.
[16] Tippmann H F. Analysis for free:Comparing programs for sequence analysis[J]. Briefings in Bioinformatics, 2004, 5(1):82-87.
[17] Rozas J, Rozas R. DnaSP, DNA sequence polymorphism:an interactive program for estimating population genetics parameters from DNA sequence data[J]. Bioinformatics, 1995, 11(6):621-625.
[18] Excoffier L, Laval G, Schneider S. Arlequin (version 3.0):An integrated software package for population genetics data analysis[J]. Evolutionary Bioinformatics, 2005, 1:47-50.
[19] Dupanloup I, Schneider S, Excoffier L. A simulated annealing approach to define the genetic structure of populations[J]. Molecular Ecology, 2002, 11(12):2571-2581.
[20] Tamura K, Stecher G, Peterson D, et al. MEGA6:Molecular evolutionary genetics analysis version 6.0[J]. Molecular Biology and Evolution, 2013, 30(12):2725-2729.
[21] Drummond A J, Suchard M A, Xie D, et al. Bayesian phylogenetics with BEAUti and the BEAST 1.7[J]. Molecular Biology and Evolution, 2012, 29(8):1969-1973.
[22] Rambaut A, Drummond A J. Tracer v1.4[J]. Encyclopedia of Atmospheric Sciences, 2007, 141(3567):2297-2305.
[23] Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences[J]. Journal of Molecular Evolution, 1980, 16(2):111-120.
[24] Felsenstein J. Confidence limits on phylogenies:An approach using the bootstrap[J]. Evolution, 1985, 39(4):783-791.
[25] Lan H, Shi L M. The origin and genetic differentiation of native breeds of pigs in southwest China:An approach from mitochondrial DNA polymorphism[J]. Biochemical Genetics, 1993, 31(1-2):51-60.
[26] Cheng Q Q, Gu Q H, Fang D D, et al. Comparison of population genetic structure of two co-distributed cyprinid species[J]. Journal of Fisheries of China, 2017, 41(8):1169-1182.[程清清, 顾钱洪, 方冬冬, 等. 同域分布两种鲤科鱼类种群遗传结构的比较[J]. 水产学报, 2017, 41(8):1169-1182.]
[27] Fang D D, Gu Q H, Zhou C J, et al. Genetic diversity of hemiculter leucisculus in fountainhead area of Huaihe River[J]. Fisheries Science, 2018, 37(5):665-673.[方冬冬, 顾钱洪, 周传江, 等. 淮河源区䱗群体遗传多样性研究[J]. 水产科学, 2018, 37(5):665-673.]
[28] González-Astorga J, Núñez-Farfán J. Effect of habitat fragmentation on the genetic structure of the narrow endemic Brongniartia vazquezii[J]. Evolutionary Ecology Research, 2001, 3(7):861-872.
[29] Harrison R. Molecular changes at speciation[J]. Annual Review of Ecology and Systematics, 1991, 22(1):281-308.
[30] Freeland J R. Molecular Ecology[M]. Chichester:John Wiley & Sons, 2005:112-116.
[31] Liu H F, Zhang C, Wang J, et al. Genetic diversity of wild Zacco platypus populations among four river systems in Henan Province analyzed by mitochondrial COI gene[J]. Journal of Fishery Sciences of China, 2018, 25(2):269-277.[刘慧芬, 张超, 王静, 等. 河南境内四水系宽鳍鱲野生群体的遗传多样性[J]. 中国水产科学, 2018, 25(2):269-277.]
[32] Li Q. Multilocus genetic diversity and population structure for the Odontobutis distributed in Yangtze river basin and Huai River[D]. Shanghai:Shanghai Ocean University, 2015.[李强. 基于多基因位点的长江流域及淮河流域沙塘鳢属鱼类遗传多样性与种群遗传结构分析[D]. 上海:上海海洋大学, 2015.]
[33] Li C. Study on overground river of Ergu River in Northern Song Dynasty[J]. Historical Geography, 2013, 12(2):57-70.[李丞. 北宋二股河地上河问题研究[J]. 历史地理, 2013, 12(2):57-70.]
[34] Ye Q C. Study on the erosion environmental development orientation in the middle Yellow River[J]. Yellow River, 1996(11):11-15.[叶青超. 黄河下游地上河发展趋势研究[J]. 人民黄河, 1996(11):11-15.]
[35] Zhang Y Y, Li Z L, Liu X J. Evolution of interconnected river and lake networks in the Huai River Basin over the last millennium[J]. South-to-North Water Transfers and Water Science & Technology, 2016, 14(4):77-83.[张永勇, 李宗礼, 刘晓洁. 近千年淮河流域河湖水系连通演变特征[J]. 南水北调与水利科技, 2016, 14(4):77-83.]
[36] Jin M. Research on the urbanization response of interconnected river system network in Zhengzhou City[D]. Zhengzhou:Zhengzhou University, 2014.[靳梦. 郑州市水系连通的城市化响应研究[D]. 郑州:郑州大学, 2014.]
[37] Yu L. Research on the river system form structure and connectivity of Huaihe river basin[D]. Zhengzhou:Zhengzhou University, 2017.[于璐. 淮河流域水系形态结构及连通性研究[D]. 郑州:郑州大学, 2017.]
[38] Gao Y, Wang S Y, Guo J. Quaternary palaeoenvironmental oscillations drove the evolution of the Eurasian Carassius auratus complex (Cypriniformes, Cyprinidae)[J]. Journal of Biogeography, 2012, 39(12):2264-2278.
[39] Flanagan S P, Rose E, Jones A G. Population genomics reveals multiple drivers of population differentiation in a sex-role-reversed pipefish[J]. Molecular Ecology, 2016, 25(20):5043-5072.
[40] Shen Y P, Kang J C. A 150 ka climatic record of loess from grain-size data in Linxia, Gansu[J]. Arid Land Geography, 1992, 15(3):44-49.[沈永平, 康建成. 十五万年来临夏黄土粒度变化的气候记录[J]. 干旱区地理, 1992, 15(3):44-49.]
服务与反馈:
Html全文阅读】【文章下载】【发表评论】【查看评论】【加入收藏
提示:查看此文需要支付$0.00
关于我们  |  联系我们  |  期刊介绍  |   在线留言
Copyright  ©  2009 中国水产科学杂志
京ICP备09074735号-7
京公网安备1101060260001号