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饲喂乳酸菌对凡纳滨对虾幼虾肉质的影响
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作者:夏雨1  易华西1  范荣波2  张冬1  梁晶晶3  蔡玉勇3  张兰威1 
单位:1. 中国海洋大学食品科学与工程学院, 山东 青岛 266003;
2. 青岛农业大学海都学院, 山东 烟台 265100;
3. 青岛根源生物技术集团有限公司, 山东 青岛 266000
关键词:凡纳滨对虾幼虾 乳酸菌 肉质 脂肪酸 肌肉质构 
分类号:S965
出版年·卷·期(页码):2020·27·1(74-81)
摘要:
在凡纳滨对虾(Litopenaeus vannamei)幼虾饲料中添加3株植物乳杆菌[Lactobacillus plantarum YRL45、Lactobacillus plantarum QL、Lactobacillus plantarum KTP(C-2)]和3株副干酪乳杆菌(Lactobacillus paracasei M5、Lactobacillus paracasei X12、Lactobacillus paracasei SB27),采用质构仪TPA模式和气相色谱法分析喂养后肌肉质构和脂肪酸含量变化,研究乳酸菌对凡纳滨对虾幼虾肌肉品质的影响。结果表明,添加乳酸菌能改善凡纳滨对虾幼虾肌肉的弹性和咀嚼性,其中植物乳杆菌[YRL45、QL、KTP(C-2)]的改善效果最好,将幼虾肌肉的弹性和咀嚼性分别提高了35.14%和85.71%(P>0.05)。副干酪乳杆菌(M5、X12、SB27)能提高对虾肌肉持水性并且能显著降低对虾肌肉中饱和脂肪酸含量,提高多不饱和脂肪酸含量,其中棕榈酸、十七烷酸和硬脂酸含量显著降低,EPA与DHA的含量分别增加了23.22%和34.40%。综上所述,在饲料中添加植物乳杆菌[YRL45、QL、KTP(C-2)]对凡纳滨对虾幼虾肌肉弹性和咀嚼性有改善作用,副干酪乳杆菌(M5、X12、SB27)能显著改善凡纳滨对虾幼虾肌肉的脂肪酸组成。
Litopenaeus vannamei is one of the most important aquaculture products in China. In this study, Lactobacillus plantarum[YRL45, QL, and KTP (C-2)] and Lactobacillus paracasei (M5, X12, and SB27) were added to the feed of juvenile Litopenaeus vannamei for 4 weeks, to study the effects of lactic acid bacteria on the muscle quality of juvenile L. vannamei. TPA analysis was used to evaluate the texture of the muscle of the shrimp, while the content of fatty acids in the muscle was analyzed using gas chromatography. The results showed that the addition of lactic acid bacteria improved the muscle elasticity and chewiness (P>0.05). The positive effects of L. plantarum[YRL45, QL, and KTP (C-2)] outweighed those of L. paracasei, with the elasticity and chewiness of the muscles increasing by 35.14% and 85.71% (P>0.05), respectively. Lactobacillus paracasei (M5, X12, and SB27) increased the water holding capacity (P>0.05) and polyunsaturated fatty acids content of the shrimp muscle (P<0.05), while the content of saturated fatty acids in the muscle was significantly reduced (P<0.05). In the Lactobacillus paracasei group, the contents of palmitic acid, heptadecanoic acid, and stearic acid were significantly reduced (P<0.05), whilst the contents of EPA and DHA increased by 23.22% and 34.40% (P>0.05), respectively. In this study, Lactobacillus plantarum (YRL45, QL, and KTP (C-2)) and Lactobacillus paracasei (M5, X12, and SB27) caused an improvement in the muscle elasticity, chewiness, and water holding capacity of shrimp, which may be related to the improvement of the muscle antioxidant capacity and protein quality. Lactobacillus plantarum and Lactobacillus paracasei slightly regulated the basic nutrient content of the shrimp muscle. Lactobacillus paracasei significantly improved the fatty acid composition of the shrimp muscle, which may be due to the regulation of the nutrition and flora of the shrimp or production of nutrients to improve the fatty acid composition of the shrimp. However, the specific mechanism for this requires further study. In summary, this study showed that Lactobacillus plantarum[YRL45, QL, and KTP (C-2)] had superior effects on muscle elasticity and chewiness of juvenile Litopenaeus vannamei, while Lactobacillus paracasei (M5, X12, and SB27) improved the fatty acid composition in the shrimp muscle. This indicates that lactic acid bacteria can improve muscle quality and fatty acid composition of juvenile Litopenaeus vannamei. Furthermore, this provides a theoretical basis for the application of probiotics in the culture of juvenile Litopenaeus vannamei.
该文献标准引用格式:
XIA Yu, YI Huaxi, FAN Rongbo, ZHANG Dong, LIANG Jingjing, CAI Yuyong, ZHANG Lanwei.Effects of dietary lactic acid bacteria on the meat quality of juvenile Litopenaeus vannamei[J].Journal of Fishery Sciences of China,2020,27(1):74-81.[夏雨, 易华西, 范荣波, 张冬, 梁晶晶, 蔡玉勇, 张兰威.饲喂乳酸菌对凡纳滨对虾幼虾肉质的影响[J].中国水产科学,2020,27(1):74-81.]
参考文献:
[1] Wu X Y, Liang M Q, Xue C H, et al. Effects of salt addition in feed on muscle nutritional composition and texture of Litopenaeus vannamei cultured in low salinity water[J]. Marine Fisheries Research, 2008, 29(6):84-89.[吴新颖, 梁萌青, 薛长湖, 等. 饲料中添加盐对低盐养殖凡纳滨对虾肌肉常规营养成分及质构的影响[J]. 海洋水产研究, 2008, 29(6):84-89.]
[2] Gou N N, Wang K F, Yang X C. Review of salinity levels and dietary protein on nutrition and digestion of litopenaeus vannamei[J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2018, 27(3):306-315.[苟妮娜, 王开锋, 杨新成. 盐度和饲料蛋白对凡纳滨对虾营养及消化作用研究进展[J]. 西北农业学报, 2018, 27(3):306-315.]
[3] Fishery Bureau of Ministry of Agriculture. China Fishery Statistics Yearbook[M]. Beijing:China Agriculture Press, 2018.[农业部渔业局. 中国渔业统计年鉴[M]. 北京:中国农业出版社, 2018.]
[4] Li Z Q, Du Z J, Fan L J. Effects of feeds and nutrition on savour and flavour of aquati products[J]. Fisheries Science, 2002, 21(2):38-41.[李志琼, 杜宗君, 范林君. 饲料营养对水产品肉质风味的影响[J]. 水产科学, 2002, 21(2):38-41.]
[5] Li B A. Study on flesh quality of large yellow croaker (Pseudosciaena crocea) and tilapia (Oreochramis miloticas×O.aureus)[D]. Shanghai:Shanghai Ocean University, 2015.[李百安. 大黄鱼(Pseudosciaena crocea)、罗非鱼(Oreochramis miloticas×O.aureus)的肌肉品质研究[D]. 上海:上海海洋大学, 2015.]
[6] Liu B, Leng X J, Li X Q, et al. Effect of eucommia ulmoides on growth, serum non-specific immunity, and muscle composition of Litopenaeus vannamei[J]. Journal of Fishery Sciences of China, 2013, 20(4):869-875.[刘波, 冷向军, 李小勤, 等. 杜仲对凡纳滨对虾生长、血清非特异性免疫和肌肉成分的影响[J]. 中国水产科学, 2013, 20(4):869-875.]
[7] Ezquerra Brauer J M, Salazar Leyva J A, Bringas Alvarado L, et al. Effect of dietary protein on muscle collagen, collagenase and shear force of farmed white shrimp (Litopenaeus vannamei)[J]. European Food Research and Technology, 2003, 217(4):277-280.
[8] Ray A J, Leffler J W, Browdy C L. The effects of a conventional feed versus a fish-free feed and biofloc management on the nutritional and human sensory characteristics of shrimp (Litopenaeus vannamei)[J]. Aquaculture International, 2019, 27(1):261-277.
[9] Liao X D, Wu R J, Ma G, et al. Effects of Clostridium butyricum on antioxidant properties, meat quality and fatty acid composition of broiler birds[J]. Lipids in Health and Disease, 2015, 14:36-44.
[10] Zhou X J, Jin E H, Li S H, et al. Effects of dietary supplementation of probiotics (Bacillus subtilis, Bacillus licheniformis, and Bacillus natto) on broiler muscle development and meat quality[J]. Turkish Journal of Veterinary and Animal Sciences, 2015, 39:203-210.
[11] Liu Y H, Li Y Y, Feng X C, et al. Dietary supplementation with Clostridium butyricum modulates serum lipid metabolism, meat quality, and the amino acid and fatty acid composition of Peking ducks[J]. Poultry Science, 2018, 97(9):3218-3229.
[12] Sha Y J, Wang L, Liu M, et al. Effects of lactic acid bacteria and the corresponding supernatant on the survival, growth performance, immune response and disease resistance of Litopenaeus vannamei[J]. Aquaculture, 2016, 452:28-36.
[13] Cui Y, Lin X D, Kang M L, et al. Effect of high hydrostatic pressure combined with freezing on the shucking and quality of Penaeus vannamei[J]. Modern Food Science and Technology, 2018, 34(10):171-178.[崔燕, 林旭东, 康孟利, 等. 超高压协同冷冻脱壳对南美白对虾品质的影响[J]. 现代食品科技, 2018, 34(10):171-178.]
[14] Wang C, Wang H, Li X, et al. Effects of oxygen concentration in modified atmosphere packaging on water holding capacity of pork steaks[J]. Meat Science, 2019, 148:189-197.
[15] National Health and Family Planning Commission of PRC. GB 5009.3-2016. Determination of Moisture in Foods[S]. Beijing:Standards Press of China, 2017.[中华人民共和国国家卫生和计划生育委员会. GB 5009.3-2016. 食品中水分的测定[S]. 北京:中国标准出版社, 2017.]
[16] National Health and Family Planning Commission of PRC. GB 5009.4-2016. Determination of ash in foods[S]. Beijing:Standards Press of China, 2017.[中华人民共和国国家卫生和计划生育委员会. GB 5009.4-2016. 食品中灰分的测定[S]. 北京:中国标准出版社, 2017.]
[17] National Health and Family Planning Commission of PRC. GB 5009.5-2016. Determination of protein in foods[S]. Beijing:Standards Press of China, 2017.[中华人民共和国国家卫生和计划生育委员会. GB 5009.5-2016. 食品中蛋白质的测定[S]. 北京:中国标准出版社, 2017.]
[18] Ouraji H, Abedian Kenari A M, Shabanpour B, et al. Growth response and muscle lipid quality of Indian white shrimp fed different oils at two dietary lipid levels[J]. Journal of Food Quality, 2010, 33(4):405-423.
[19] Folch J, Lees M, Sloane Stanley G H. A simple method for the isolation and purification of total lipids from animal tissues[J]. Journal of Biological Chemistry, 1957, 226:497-509.
[20] Oswald A T O, Ishikawa M, Koshio S, et al. Nutritional evaluation of Nannochloropsis powder and lipid as alternative to fish oil for kuruma shrimp, Marsupenaeus japonicus[J]. Aquaculture, 2019, 504:427-436.
[21] Liu P. Effect of low temperature plasma on black resistance and freshness quality of Penaeus vannamei[D]. Zhoushan:Zhejiang Ocean University, 2018.[刘品. 低温等离子体对南美白对虾防黑变及品质的影响研究[D]. 舟山:浙江海洋大学, 2018.]
[22] Hao S X, Deng J C, Lin W L, et al. Effect of frozen temperature on quality of Penaeus vannamei during frozen storage[J]. Modern Food Science and Technology, 2013, 29(9):2086-2089, 2299.[郝淑贤, 邓建朝, 林婉玲, 等. 冻结速率对凡纳滨对虾虾仁贮藏特性的影响[J]. 现代食品科技, 2013, 29(9):2086-2089, 2299.]
[23] Zeng D. The study of isolation, biological characteristics and mechanism of probiotics from Cyprinus carpio[D]. Ya'an:Sichuan Agricultural University, 2009.[曾东. 鲤益生菌的筛选、生物学特性及作用机理的研究[D]. 雅安:四川农业大学, 2009.]
[24] Cheng Y F, Chen Y P, Du M F, et al. Evaluation of dietary synbiotic supplementation on growth performance, muscle antioxidant ability and mineral accumulations, and meat quality in late-finishing pigs[J]. Kafkas Universitesi Veteriner Fakultesi Dergisi, 2018, 24(5):673-679.
[25] Wang G X, Huang Y H, Huang W Q, et al. Effects of lactic acid bacteria on growth, feed utilization and body composition of Penaeus vannamei[J]. China Feed, 2010(12):24-26, 32.[王国霞, 黄燕华, 黄文庆, 等. 乳酸菌对南美白对虾生长、饲料利用和体组成的影响[J]. 中国饲料, 2010(12):24-26, 32.]
[26] Lin H Z, Li Z J, Guo Z X, et al. Effects of dietary probiotics on growth and biochemical composition of whole body of juvenile shrimp, Litopenaeus vannamei[J]. South China Fisheries Science, 2008, 4(6):95-100.[林黑着, 李卓佳, 郭志勋, 等. 益生菌对凡纳滨对虾生长和全虾营养组成的影响[J]. 南方水产, 2008, 4(6):95-100.]
[27] Wang Y B, Fu L L, Lin J D. Probiotic (Bacillus coagulans) cells in the diet benefit the white shrimp Litopenaeus vannamei[J]. Journal of Shellfish Research, 2012, 31(3):855-860.
[28] Madani N S H, Adorian T J, Farsani H G, et al. The effects of dietary probiotic Bacilli (Bacillus subtilis and Bacillus licheniformis) on growth performance, feed efficiency, body composition and immune parameters of whiteleg shrimp (Litopenaeus vannamei) post larvae[J]. Aquaculture Research, 2018, 49(5):1926-1933.
[29] Jia G W, Guo R, He W, et al. Fat nutritional needs of Penaeus vannamei[J]. Hebei Fishery, 2018(2):57-59.[贾高旺, 郭冉, 何伟, 等. 凡纳滨对虾的脂肪营养需求[J]. 河北渔业, 2018(2):57-59.]
[30] Yang Q H, Zhang W, Tan B P, et al. Effects of dietary n-3 HUFA on juvenile white shrimp, Litopenaeus vannamei:Growth, feed utilization, antioxidant enzymes activities and fatty acid compositions[J]. Aquaculture Research, 2019, 50(3):882-894.
[31] Balasundar A, John G, Mohamed R R, et al. Nutritional value of two bacterial strains Bacillus subtilis RCMB21 and Pseudomonas fluorescens RCMB39 as feed supplement for freshwater prawn Macrobrachium malcolmsonii[J]. Asian Journal of Animal and Veterinary Advances, 2018, 13(3):282-294.
[32] Kumar V, Roy S, Meena D K, et al. Application of probiotics in shrimp aquaculture:Importance, mechanisms of action, and methods of administration[J]. Reviews in Fisheries Science & Aquaculture, 2016, 24(4):342-368.
[33] Lobo C, Martín M V, Moreno-Ventas X, et al. Shewanella putrefaciens Pdp11 probiotic supplementation as enhancer of Artemia n-3 HUFA contents and growth performance in Senegalese sole larviculture[J]. Aquaculture Nutrition, 2018, 24(1):548-561.
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